Biotin and derivatives
IBA LifeSciences Biotin powder; 5 g
Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to the biotin-binding pockets of streptavidin, Strep-Tactin, and Strep-TactinXT with different affinities. As a result, it allows the efficient elution of Strep-tagII or Twin-Strep-tag fusion proteins from Strep-TactinXT, while maintaining the reusability of the resin. It can be used for preparation of Strep-TactinXT elution buffer, Buffer BXT. In addition, biotin can be used for elution of biotinylated proteins from Strep-Tactin, where it binds irreversibly to the resin. Hence, Strep-Tactin cannot be regenerated in this kind of application.
Sigma Aldrich Fine Chemicals Biosciences Biotin, 58-85-5, MFCD00005541, 1g
Empirical Formula (Hill Notation) C10H16N2O3S, ≥99% (HPLC), Molecular Weight 244.31, lyophilized powder, Synonym: D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H; Biotin may be used to elute proteins from avidin/streptavidin resins.
Medchemexpress LLC Cy3 NHS ester | 1393363-07-9 | 99.24% | 590.15 | 25 MG
Cy3 NHS ester is a CY dye, which stands for Cyanine. Cyanine compounds are characterized by having two nitrogen atoms connected by an odd number of methyl units. These dyes are known for their long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility, and relatively simple synthesis. CY dyes are frequently used for labeling proteins, antibodies, and small molecular compounds. Labeling protein antibodies can be achieved through a straightforward mixing reaction.
- Long wavelength
- Adjustable absorption and emission
- High extinction coefficient
- Good water solubility
- Relatively simple synthesis
- Often used for labeling proteins, antibodies, and small molecular compounds
- Protein antibody labeling can be completed through a simple mixing reaction
Medchemexpress LLC Biotin-Val-Ala-Asp(OMe)-FMK | 1135688-15-1 | ≥95.0% | 672.81 g/mol | C30H49FN6O8S | 50 MG
Biotin-VAD-FMK is a biotin-labeled, cell-permeable, irreversible pan-caspase inhibitor used to identify and label active caspases in cell lysates. It serves as an affinity probe for detection or capture of active caspases in apoptosis research and protease-profiling workflows, and is compatible with biochemical assays and pull-down experiments.
- Biotin-labeled for affinity capture and detection.
- Cell-permeable, irreversible caspase inhibitor.
- Enables identification and labeling of active caspases in cell lysates.
- Compatible with biochemical assays, protease profiling, and pull-down workflows.
- Supplied as a solid with defined molecular weight and formula for experimental planning.
Medchemexpress LLC DBCO-NHS ester 3 100mg | 1393350-27-0 | 416.43 | C24H20N2O5 | 100 MG
DBCO-NHS ester 3 is an amine-reactive dibenzocyclooctyne (DBCO)-based N-hydroxysuccinimide (NHS) ester used as a clickable linker for constructing antibody-drug conjugates and for labeling amine-containing biomolecules via strain-promoted alkyne-azide cycloaddition. Supplied as a 100 MG solid for research use.
- Amine-reactive NHS ester enables covalent coupling to primary amines.
- DBCO moiety supports copper-free click chemistry (SPAAC) with azides.
- Suitable for antibody-drug conjugate linker synthesis and protein labeling.
- Provided in small research-scale solid quantities for laboratory workflows.
- Molecular formula C24H20N2O5; molecular weight 416.43 g/mol.
Medchemexpress LLC Biotin-PEG3-acid 250mg | 252881-76-8 | 447.55 g·mol⁻¹ | C19H33N3O7S | 250 MG
Biotin-PEG3-acid is a biotin-labeled PEG3 linker with a terminal carboxylic acid, supplied as a solid for use in conjugation and PROTAC linker synthesis. It provides a hydrophilic spacer arm to attach biotin to small molecules or probes, enabling affinity capture, purification, and detection workflows.
- Biotin-labeled PEG3 linker with carboxylic acid terminus.
- Suitable for coupling chemistries such as amide bond formation.
- Used in PROTAC synthesis, biotinylation, pull-downs, and detection assays.
- Molecular weight 447.55 g·mol⁻¹ and CAS 252881-76-8.
- Purity 98.0%.
- Supplied as a 250 MG solid; other sizes available.
Medchemexpress LLC Norbornene-NHS | 1888335-48-5 | C12H13NO5 | 50 MG
Norbornene-NHS is a click chemistry reagent and a dienophile for conjugation via copper-free Click reaction with tetrazines. It is for research use only and not sold to patients.
- Purity: 92.08%
- Molecular weight: 251.24
- Appearance: Liquid
- Color: Light yellow to yellow
- Used for conjugation via copper-free click reaction
Medchemexpress LLC Biotin-PEG3-acid 1g | 252881-76-8 | 447.55 | C19H33N3O7S | 1 G
Biotin-PEG3-acid is a biotin-labeled, PEG-based PROTAC linker used to introduce a biotin tag with a short PEG3 spacer in PROTAC synthesis and bioconjugation workflows. The material is supplied as a 1 g purified solid (typical purity 98.0%) with molecular weight 447.55 g/mol and chemical formula C19H33N3O7S, and is accompanied by a batch-specific certificate of analysis.
- Provides a biotin handle separated by a PEG3 spacer to reduce steric hindrance
- Designed for incorporation into PROTACs and other bioconjugates
- Improves aqueous solubility compared with direct biotinylation
- Typically supplied with batch certificate showing ~98.0% purity
- Solid form with known molecular weight and formula for formulation planning